Further observations on the formolether concentration technique for faecal parasites. Further observations on the formolether concentration. Formaldehyde commercial product 1200mls na2hpo4 10. Oct 29, 20 clonorchiasis is a chronic neglected disease caused by a liver fluke, clonorchis sinensis. The oocysts of isospora belli can be demonstrated in faeces after formol ether concentration. Dec 23, 2010 this six minute video presents the formol ether concentration technique used to examine fecal parasites. Formalin ether concentration technique is a method use to concentrate parasitic elements through sedimentation to enhance recovery. Bukumbi for intestinal parasitic infections with three diagnostic methods. Disadvantagesoftheformalin ether technique include i concentration ofgranular material, ii suboptimal concentration of h. Since the introduction of ritchies method in 1948, many laboratories have chosen the formalin ether concentration procedure for parasitological exams in fecal specimens. Evaluation performance of diagnostic methods of intestinal. The formolether concentration technique for intestinal. Pdf a modified formalinether concentration technique for. Evaluation of the efficacy of the mini parasep sf faecal.
More than 10 faecal examination techniques are known, including direct smear ds andor concentration tada et al. Pdf we compared the efficacy and applicability of a modified formalinether concentration technique mfect to the conventional fect cfect and the. A total of 120 stool samples were collected, 60 male and 60 female from fepeaury to april 2014. Time delays between patient and laboratory selectively. Formal ether sedimentation technique for the concentration. A semi structured interviewer administered questionnaire was used to collect information from participants.
We assessed the diagnostic accuracy of the katokatz method and the formalin ether concentration technique fect for c. Miranda questions for research update brief summary draw and label. This resource was developed by charles adjei osei of the kwame nkrumah university of. An accurate diagnosis is important in order to identify individuals and communities in need of intervention, and for monitoring drug efficacy and potential emergence of resistance. A modified formalin ether concentration technique for diagnosis of human strongyloidiasis article pdf available in the korean journal of parasitology 516. To compare the routine and concentration techniques. A total of 354 faecal specimens were collected from students in northwest ethiopia and screened with katokatz. In developed countries the formol ether technique is the concentration method of choice.
A comparative study of concentration techniques for. Formol ether concentration method in the diagnosis of active schistosoma mansoni in patients with detectable iha. Miniflotac, a new tool for copromicroscopic diagnosis of. Under diagnosis of intestinal schistosomiasis in a. This study was conducted to assess for any significant difference in the use of either modified katokatz technique or formolether concentration technique in diagnosing intestinal helminth infection from dogs faeces. The first page of the pdf of this article appears above. Comparison of sucrose flotation, zinc sulphate znso 4 flotation and formol ether fe sedimentation techniques revealed that fe was the most effecient of the three p concentration of c. Modified formalinether concentration technique for diagnosis of.
Formal ether concentration technique 20142015 abstract. Intestinal parasitic infection and assessment of risk. Comparison of the katokatz, wet mount, and formolether concentration diagnostic techniques for intestinal helminth infections in ethiopia. The standard ridleyallen formol ether concentration use as by htd follows the method as described as in reference 1. A total of 280 stool samples were collected and only 5% of the patients were positive for ova of schistosoma mansoni in the routine direct wet mount microscopy. Faecal samples collected randomly from individualowning dogs from ileife between january and september, 2005, were transported to the laboratory. Chemotherapy is the mainstay of control and treatment efficacy is usually determined by microscopic examination of fecal samples. This study was aimed to assess the prevalence and associated factors of parasitic infections among patients.
Although several diagnostic methods such as katokatz and formol ether concentration fec techniques are available, direct wet mount is the commonly used as a reliable diagnosis method for the diagnosis of intestinal parasitic infections generally in. The aim of this study was to assess the prevalence and determinant factors of anemia in. Results were shown in tables, percent and prevalence rates. The aim of this study was to evaluate the diagnostic performance of direct wet mount microscopy compared to formalin ether concentration fec technique in detecting intestinal helminths in pregnant women. Centrifuge time should be calculated after centrifuge reaches full speed. Parasitic elements are concentrated through sedimentation to enhance the recovery in fecal specimens. Apr 12, 2018 formol ether sedimentation technique article available in pdf the use of concentration techniques increases the chances of identifying intestinal parasitic wether helminths or protozoans. Institution based crosssectional study design was used. Determinant factors of anaemia among pregnant women attending. Laboratory stack technical laboratory procedure guides for clinical laboratory scientists and students including the latest technology and innovations in laboratory science. Fecal concentration is a routine part of the ova and parasite examination and allows the detection of small numbers of organisms that may be missed by using a direct wet smear.
Principle parased is a closed concentration system for the recovery of eggs, larvae, and protozoa from preserved fecal specimens. Fecal samples of dogs 100 and cats 100 were collected and subjected to. We compared the diagnostic accuracy of a further modified version of the recently developed flotac technique with that of the widely used formalin ether concentration technique fect. Comparison of the flotac400 dual technique and the formalin. Jul 17, 2019 anaemia is a low blood haemoglobin concentration and has been shown to be a public health problem affecting both developing and developed countries. A total of 103 samples collected from the same number of individuals were examined for intestinal parasites using the direct smear method and the formol ether concentration technique. The children were categorized into three age groups 68 represent 41. Comparison of the katokatz method and etherconcentration. A cross sectional study was conducted on 364 patients, attending shahura health center, northwest ethiopia. Further observations on the formol ether concentration technique for faecal parasites.
In particular, the formalin ether sedimentation technique 406th medical general laboratory mgl, first reported by ritchie 1948, is commonly used for detecting helminth eggs, larvae, and. Technical methods further observations on the formol ether concentration technique for faecal parasites a. Ether concentration technique in diagnosis of intestinal parasites. The formolether sedi mentation technique is a concentration technique that is widely used. In the latter method the heavier ova schistosoma, fasciola fail to rise to thesurface. Jcdr concentration techniques, simple salt floatation concentration techniques like simple slat flotation, zinc sulphate centrifugal floatation, formol ether concentration and modified formol ether concentrationesults the prevalence of the intestinal parasitic infections was higher in the rural populationet price. This technique yieldsa cleaner preparation and morphology of the parasites than the sedimentation procedure garcia, 2001, melvin and brooks, 1985. The formalinether concentration technique fect is a widely used sedimentation technique for the diagnosis of. Pdf the formolether concentration technique for intestinal. Diagnostic performance of direct wet mount microscopy in. Principle of formal ether formalinethyl aceatate sedimentation technique. The most common intestinal nematodes of dogs are toxocara canis, hookworm and trichuris vulpis. J clin pathol online june 16, 2011 preservative used 28% 26% 23% 1% 1% 21% formalin in water formalin in saline.
The formalin ether concentration technique fect is a widely used sedimentation technique for the diagnosis of intestinal protozoa in preserved stool samples. Comparison of the flotac400 dual technique and the. They showed that the formol ether concentration technique was more sensitive as compared to the other methods. This six minute video presents the formol ether concentration technique used to examine fecal parasites. Vernier measurements should be taken if an unusual parasite is identified or if a confirmation is required. Comparison of the katokatz, wet mount, and formolether. Two types of concentration techniques are used for stool examination i sedimentation ii floatation i sedimentation technique formol ether technique formol ether saf formol ether pva ii floatation technique zinc sulfate saturated salt solution. Stool concentration techniques, especially formol formalin ether or formol ethyl acetate sedimentation concentration is the preferred method for detection of helminth eggs and protozoa cysts in many clinical parasitology laboratories, although to a lesser extent in africa and asia.
The superiority of formol ether concentration over the well known zinc sulphate flotation method was demonstrated by ritchie,pan,and hunter 1952. The present study was aimed to validate a new copromicroscopic technique, the miniflotac and to compare its diagnostic efficiency and sensitivity with four other copromicroscopic techniques. Formal ether sedimentation stool concentration technique. We compared the technique, using a single stool sample and a commercial preparation kit, with three tests traditionally used in the endemic countries katokatz thick smear. Direct wet smear technique like lugol stain is quick to prepare and inexpensive when compared with other methods but it can miss eggs of hookworms if concentration is too low 12. Alternatively, they can be seen in a faecal smear stained by modified ziehlneelsen where the oocysts stain a granular red colour against a green background. Sedimentation methods use centrifugation to concentrate the protozoa, helminth ova and. Jcdr concentration techniques, simple salt floatation. Formol ether concentration sedimentation technique allen and ridely modification. Get a printable copy pdf file of the complete article 319k, or click on a page image below to browse page by page.
According to ritchie 1948, the recovery efficiency of the mgl technique for. Pregnant women are the most vulnerable groups to anaemia due to several factors, including parasitic infection and feeding habits during their pregnancy. Formaldehyde preserved stool samples from 108 individuals in co. The sedimentation technique used at cdc is the formalinethyl acetate technique, a diphasic sedimentation technique that avoids the problems of flammability of ether, and which can be used with specimens preserved in formalin, mif or saf. Stool specimens were collected and examined using formol ether concentration technique. Fecal parasite examination the formolether concentration. In such cases, the use of concentration techniques increases the chances of detecting parasitic organisms, thus increasing the sensitivity of copromicroscopic techniques. Pdf comparison of routine and concentration techniques. The total prevalence of intestinal helminths was 18. The formalinether concentration technique is typically conducted in reference laboratories for the concurrent diagnosis of helminth and intestinal protozoa. Using a stick, an estimated 1 g peasize of representative faeces was emulsified in about 4 ml of 10% formol water contained in a screwcap tube. Sedimentation techniques use solutions of lower specific gravity than the parasitic organisms, thus concentrating the latter in the sediment.
Stool samples were collected processed with formol ether concentration technique and examined for viable eggs of ascaris. Formalin ether concentration technique laboratory stack. Strongyloides stercoralis, formalin ether concentration technique, agar plate culture, feces, diagnosis in tropical and subtropical areas, strongyloidiasis is a widespread human parasitic disease that can be lifethreatening to immunocompromised hosts as well as patients undergoing corticosteroid treatment or chemotherapy 1. The formol ether concentration fec technique ridley and hawgood, 1956 is also widely used and has the advantages of fixing the parasites, thus rendering the samples noninfectious, as well as preserving many types of cysts, which the kk technique does not cheesbrough, 2005.
This method increases the sensivity as compare to direct wet mount with dobell iodine or normal saline. Formal ether sedimentation technique for the concentration of stool parasites formal ether sedimentation technique for the concentration of stool parasites. The efficacy of mini parasep, a new faecal parasite concentrator developed by the company diasys europe limited formerly intersep ltd was evaluated alongside the modified formol ether concentration and direct smear techniques using 120 stool samples in calabar, nigeria between may and june, 2011. Comparison of katokatz and formolether concentration. Nov 23, 20 soiltransmitted helminth infections are a major public health problem. Formolether concentration method in the diagnosis of. Comparison formalinethyl ether sedimentation, formalinethyl.
Dec 26, 2015 therefore, the aim of this study was the evaluation performance of diagnostic methods of intestinal parasitosis in school age children in ethiopia. This study was conducted to assess for any significant difference in the use of either modified katokatz technique or formol ether concentration technique in diagnosing intestinal helminth infection from dogs faeces. With the 50 ml centrifuge vial still loosely attached to the filter unit loose attachment will facilitate the release of air pressure during use, insert the open en d of the filter unit into the specimen vial until the. The formolether concentration technique for intestinal parasites. The study was designed at state elementary school 27 peusangan, bireuen. Modified formalinether concentration technique for diagnosis. International journal of current microbiology and applied. Evaluation and modification of the formalinether sedimentation. We compared the efficacy and applicability of a modified formalinether concentration technique mfect to the conventional fect cfect. Stool concentration techniques, especially formol formalinether or formolethyl acetate sedimentation concentration is the preferred method for detection of helminth eggs and protozoa cysts in many clinical parasitology laboratories 79, although to a lesser extent in africa and asia. A comparative study of concentration techniques for detection.
Laboratory procedures for stool examination laboratory. We compared the accuracy of the katokatz method and ether concentration technique for the diagnosis of soiltransmitted helminth infections. Jcdr concentration techniques, simple salt floatation, zinc. The most commonly used fixatives for stool preservation are either formalin or sodium acetateacetic acidformalin saf 3, 24, 40. Four layers formed after the second centrifugation of the fact improved diagnosis of strongyloides stercoralis using recombinant antigenbased. Al mofarreh polyclinic, kkuh, riyadh, saudi arabia 2 department of medicine, kkuh, riyadh, saudi arabia click here for correspondence address. The inclusion of two or three different concentration techniques with different principles into the routine diagnostic tests increased the sensitivity. The manufacturers instruction was strictly followed in the evaluation of. Formol ethyl acetate sedimentation concentration is a technique for detecting eggs in stool by microscopy, but has not to our knowledge been evaluated for s. To evaluate a formol acetone concentration method in comparison with the conventional direct iodine preparation and formol ether concentration methods. However, taking into account the acknowledged dangers caused by exposure to ether and formaldehyde, research was initiated to develop techniques as efficient as ritchies method, 14. There is a need for accurate diagnosis of intestinal parasite infections in humans, but currently available copromicroscopic techniques have shortcomings, such as low sensitivity. Formol ether concentration technique is taken as a gold standard method to detect most intestinal parasites. The fec study on a total of 80 100% elementary students showed that 12 15% sample had the sth.
The technique is appropriate for stool parasites, especially trematode. The formolether method is a widely used technique for stool examination. Mar 31, 2011 this video demonstrates both the sedimentation formol ether and flotation zinc sulfate methods of stool feces concentration. Comparison of staining and concentration techniques for. Comparison of two techniques for diagnosis of intestinal. Pdf comparison of the katokatz, wet mount, and formol. Ether concentration techniques to obtain the number of. Adaptation of ritchies method for parasites diagnosing. The technique recommended for the qualitative diagnosis of intestinal parasites both helminths and intestinal protozoa is the formol ether concentration method fecm 7,8, 9 performed on three. Ridley fromthe hospitalfor tropical diseases, stpancras way,london thesimplified version ofritchies formol ether technique for the concentration offaecal cysts, ova, and larvae ridley and hawgood, 1956, which is no. The overall prevalence of ascaris infection found in this. August 3, 2012 acharya tankeshwar lab diagnosis of parasitic disease, parasitology 7. A cross sectional study was conducted from may to june 20. Under diagnosis of intestinal schistosomiasis in a referral.
Mix the contents of the vial by shaking vigorously or vortexing for 30 seconds. Stool samples were examined using formol ether concentration and katokatz thick smear techniques. Diethyl ether commercial product, pml 10% neutral buffered formalin ph 7. Therefore, formol ether concentration technique should be adopted for routine faecal examination since it exposes a higher percentage of infection missed by direct smear method, which is the implemented method by medical diagnostic laboratories and hospitals in developing countries e. This video demonstrates both the sedimentation formol ether and flotation zinc sulfate methods of stool feces concentration. Mohammad a al mofarreh 1, ahmad m al akwaa 2, sayed w hasan 1, ibrahim a al mofleh 2 1 dr. Single stool sample was processed for direct, formol ether concentration fec and kato katz methods. Formal ether sedimentation technique for the concentration of.
Clonorchiasis is a chronic neglected disease caused by a liver fluke, clonorchis sinensis. Formol ether concentration method is the sedimentation technique normally used for detecting parasite infections, especially eggs and protozoan cysts with a high fat in stool samples. The aim of this study was to evaluate the operational characteristics sensitivity and negative predictive value npv of wet mount, formol ether concentration fec, and katokatz techniques for the determination of intestinal parasitic infections. Low sensitivity of the formolethyl acetate sedimentation. Four layers formed after the second centrifugation of the fact improved diagnosis of strongyloides stercoralis.
The filtrate was added to 3 ml of ether and it was centrifuged at 3000 rpm for 60 seconds and allowed to settle. However, it is striking that the kato technique is not routinely used in laboratories of developed countries, not even if schistosomiasis is suspected. Most types of worm eggs including schistosomes eggs can be recovered by formol ether concentration technique. Pdf a modified formalinether concentration technique.